Figure 2
The number of the G2 cells increases prior to development of fibrosis. (a) Immunoblotting of tissue lysates from indicated samples. α-tubulin was used as the loading control. (b) Immunoblotting of tissue lysates from indicated samples. α-SMA and α-tubulin were used as markers for fibrosis and a loading control, respectively. The molecular weights (kDa) are shown in the right-hand side of the images. Arrows indicate the height of intended bands. (c) Co-immunostaining with Anti-p-Cdk1Y15 (green) and E-Cadherin (red). Scale bar, 100 µm. (d,e) Co-immunostaining with Anti-p-Cdk1Y15 (green) and Ki67 (red). Surrounded area is enlarged in (e). Scale bars, 100 µm. (f) Quantification of the number of p-Cdk1Y15 positive cells (n = 15). Data are as given averages ± SD; ***P < 0.001 (two-tailed unpaired Student’s t-test).
