Figure 1

Dose range finding study was conducted to define dose levels of ATEC or DEHP for the test with Salmonella typhimurium. (A–D) Bacterial revertant formation was measured in triplicate by Ames test. Salmonella typhimurium TA98 (A), TA100 (B), TA1535 (C) and TA1537 (D) strains were treated with ATEC or DEHP in the absence of S9 mix. The number of revertant colonies was counted manually. Data in bar graph represent mean ± SED (n = 3). When the results in experimental groups were at least twice higher than that in negative control, it was considered to be positive.