Figure 4

Cytotoxic effect of ATEC or DEHP was measured in Chinese hamster lung (CHL/IU) cells. (A–C) CHL/IU cells were treated with ATEC or DEHP without or with S9 mix for 6 h, respectively. Each well was washed with Dulbecco’s phosphate-buffered saline (D-PBS). Then, a fresh EMEM medium was added and cultured for an additional 18 h. The other group was treated with ATEC or DEHP without S9 mix for 24 h. For each group, cell suspension was mixed with trypan blue and total cell number was counted with hemocytometer. Cell viability (A), RICC (B) and RPD (C) were calculated by the methods described in materials and methods. Data in bar graph represent mean ± SED (n = 4). *p < 0.05; **p < 0.01, significant difference as compared to DEHP- or ATEC-untreated control group at each experimental condition.