Table 1 Number of revertant colonies for each bacterial strain by positive control without S9 mix.

From: Genotoxicity and glucose tolerance induction by acetyltriethylcitrate, substitute plasticizer compared to di(2-ethylhexyl)phthalate

Bacterial strain

Positive control

S9 mix

Concentration

(μg/plate)

Number of

revertants

Salmonella

typhimurium

TA98

2-Nitrofluorene (2-NF)

−

0

19 ± 5.6

1

170.3 ± 17.5*

TA100

Sodium azide (SA)

−

0

93.3 ± 2.9

0.5

474.7 ± 26.1*

TA1535

Sodium azide (SA)

−

0

7.3 ± 2.1

0.5

96.0 ± 4.4*

TA1537

9-Aminoacridine (9-AA)

−

0

4.3 ± 2.5

80

520.0 ± 55.7*

Escherichia coli

WP2uvrA

2-(2-furyl)-3-(5-nitro-2-furyl)

acrylamide (AF2)

−

0

142.0 ± 4.4

0.005

1,316 ± 46.1*

  1. Bacterial revertant formation was measured in triplicate by Ames test. Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) were treated with 2-NF, SA, and 9-AA in the absence of S9 mix. Escherichia coli (WP2uvrA(pKM101)) strains were treated with AF2 in the absence of S9 mix. The number of revertant colonies was counted manually. *When the result in experimental group was at least twice higher than that in negative control, it was considered to be positive.
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