Table 5 Chromosomal aberrations by DEHP in Chinese Hamster Lung (CHL/IU) cells.

Sample	Concentration	S9 mix	Treatment time (h)	Number of cells with structural aberrations						gap (%)
Sample	Concentration	S9 mix	Treatment time (h)	csb	ctb	cse	cte	frg	total (%)	gap (%)
DEHP (μl/ml)	0	−	6	0	0	0	0	0	0 (0.0)	0 (0.0)
	0.0325	−	6	0	0	0	0	0	0 (0.0)	0 (0.0)
	0.065	−	6	0	0	0	0	0	0 (0.0)	0 (0.0)
	0.13	−	6	0	0	0	0	0	0 (0.0)	1 (2.0)
MMC (μg/ml)	0.05	−	6	2	7	1	5	0	15* (30.0)	0 (0.0)
DEHP (μl/ml)	0	+	6	0	0	0	0	0	0 (0.0)	0 (0.0)
	0.5	+	6	0	0	0	0	0	0 (0.0)	0 (0.0)
	1	+	6	0	0	0	0	0	0 (0.0)	0 (0.0)
	2	+	6	0	0	0	0	0	0 (0.0)	0 (0.0)
CP (μg/ml)	10	+	6	0	5	0	7	0	12* (24.0)	0 (0.0)
DEHP (μl/ml)	0	−	24	0	0	0	0	0	0 (0.0)	0 (0.0)
	0.0039	−	24	0	0	0	0	0	0 (0.0)	0 (0.0)
	0.0078	−	24	0	1	0	0	0	1 (2.0)	0 (0.0)
	0.0156	−	24	0	0	0	0	0	0 (0.0)	1 (2.0)
MMC (μg/ml)	0.05	−	24	1	5	0	8	0	14* (28.0)	0 (0.0)

Number of cells with structural aberrations, chromatid break (ctb), chromatid exchange (cte), chromosome break (csb), chromosome exchange (cse), and chromatid or chromosome gap (gap) was counted under inverted microscope. *Total frequency of structural aberrations was significantly increased (>10%) in positive control groups treated with mitomycin C (MMC) or cyclophosphamide (CP).

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