Figure 2 | Scientific Reports

Figure 2

From: Efficient and specific oligo-based depletion of rRNA

Figure 2

RNA-seq-based analysis of off-target depletion. Non-rRNA transcript levels were determined in duplicates by RNA-seq following 1, 2 or 3 rounds of oligo capture and compared to non-rRNA transcript levels of an untreated control sample. Reads mapping to rRNA genes were excluded from the mRNA off-target analysis and evaluated separately. Twelve of the 13 transcripts affected by 2 rounds of oligo capture were also among the 50 transcripts affected by 3 rounds of oligo capture.

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