Figure 3

Cancer cell growth dynamics in the 3D biomimetic model. (a,c) Fold changes in cell number (relative to day 0) for MCF-7 and MDA-MB-231 in monolayer culture (2D) or within the scaffold (3D) on days 1, 3,7 and 10; percentages of cells in S and G2/M phases or in G0/G1 phases; western blot for p-MAPK (44, 42 kDa). Data represent mean ± S.D. (n = 3). (b,d) Percentages of live cells for MCF-7 and MDA-MB-231 in monolayer culture (2D) or within the scaffold (3D) on days 1, 3,7 and 10; percentages of apoptotic cells; western blot for Caspase-3 (19, 17 kDa), Caspase-9 (37, 35 kDa) and Bax (20 kDa). Data represent mean ± S.D. (n = 3).