Figure 6
From: A biomimetic 3D model of hypoxia-driven cancer progression
Hypoxia induce senescence in MDA-MB-231. (a) Inverted fluorescent microscope images and mean cell area (µm2) of MCF-7 and MDA-MB-231 in monolayer cultures (2D) or recovered after 7 days in the scaffold (3D). Staining for F-actin (phallodin, green) and nuclei stained with DAPI (blue). Scale bars: 20 µm. (b) Bright field representative images of cells positive for β-galactosidase staining (blue) in MDA-MB-231 recovered after 7 days within the 3D scaffold at different magnification. (c) Inverted microscope images of MDA-MB-231 in monolayer cultures (2D) under hypoxic (1% O2) state; mean cell area of MDA-MB-231 in monolayer cultures under normoxic (19% O2) or hypoxic (1% O2) state. Scale bar: 20 µm. Staining for F-actin (phallodin, green) and nuclei stained with DAPI (blue). (d) Percentage of MDA-MB-231 with senescent phenotype and mean cell area after 7 days of hypoxic monolayer culture (2D 1% O2) or within the scaffold (3D). (e) Confocal microscopy images of MDA-MB-231 cultured within the scaffold on day 7 in normoxic or hypoxic states (19% O2 or 1% O2 respectively). Cells are stained with DRAQ5 (red) and blue is the collagen scaffold autofluorescence. Arrow indicate cells with enlarged dimension. Scale bars: 50 µm.
