Figure 2
Effects of a high sugar diet and acetate supplementation on colonic tissue cytokines and barrier function. Colonic tissue concentrations of IL-1β (A), IL-6 (B), IL-10 (C), TNFα (D), IL-12p70 (E), and KC/GRO (F). Two days of high-sugar diet significantly decreased IL-12p70 and KC/GRO levels compared to chow fed mice. Oral acetate supplementation in a high-sugar diet elevated the concentration of IL-6 (B), IL-10 (C), and IL-12p70 (E). Barrier function following two days of a high-sugar diet as measured by the ratio of 70 kDa/4 kDa dextran movement through intestinal loops (G) and serum LPS levels (H). Mice on a high-sugar diet had an increased ratio representing a decrease in barrier function compared to chow-fed mice and increased serum LPS compared with chow, chow + acetate, and HS + acetate fed mice. Acetate supplementation normalized intestinal barrier function. Data is shown as mean ± SEM. Chow: n = 5; HS: n = 5; Chow + acetate: n = 4; HS + acetate: n = 4. **p < 0.01; *p < 0.05.
