Figure 6
High-sugar diet induces marked microbial dysbiosis. PCA plot of the microbial populations in stool samples from chow and high-sugar fed mice at day -3 (Chow: green, CH_water_0; HS: pink, HS_water_0) and after two days on the diet (Chow: purple, CH_water_2; HS: black, HS_water_2) (A). At day -3, all samples clustered together as expected. Following 2 days of a high-sugar diet (HS: black, HS_water_2), stool samples from mice on the high-sugar diet clustered separately from the chow-fed mice (Chow: purple, CH_water_2). Cecal samples in chow and high sugar fed mice after two days are shown in (B). Samples from the chow fed mice (Chow: orange, CH_water_Cec_2D) and the chow fed mice receiving acetate (Chow + Acetate: blue, CH_NaAc_Cec_2D) clustered together while stools from the high-sugar fed (HS: purple, HS_water_Cec_2D) and the high-sugar + acetate (HS + acetate: green, HS_NaAc_Cec_2D) mice also clustered together but apart from the samples from chow fed mice. Stool samples taken after two days on the diet from chow and chow + acetate mice are shown in (C). Samples from chow fed (Chow: purple, CH-water_2) and chow fed + acetate (Chow + Acetate: orange, CH_NaAc_2) clustered together. Stool samples taken after two days on the diet in high-sugar and high-sugar + acetate mice are shown in (D). Stool samples from high-sugar (HS: black, HS_water_2) and high-sugar + acetate (HS + Acetate: orange, HS_NaAc_2) fed mice also clustered together indicating that acetate did not alter the microbial changes induced by the high-sugar diet. Chow: n = 5; HS: n = 5; Chow + acetate: n = 4; HS + acetate: n = 4.
