Figure 2
Synchrotron-generated X-ray microbeam irradiation settings. (a) Optical apparatus for X-ray microbeam irradiation, using the synchrotron beamline BL-27 at the Photon Factory, High Energy Accelerator Research Organization (KEK). (b) Photo of X-ray microbeam irradiation settings with ex vivo testes organ cultures. (c) Dose profiles of the 200, 50, and 12.5 μm-width microbeams, calculated with PHITS code. The beam intensity was essentially flat within the beam width. The deviation of the dose was about ± 6% of the averaged dose. Due to the very short range of secondary electrons (1.1 μm maximum) produced by the 5.35 keV X-ray exposure, the doses delivered outside of the irradiated area was negligible (<0.25%). (d) Dose profiles of the 200, 50, and 12.5 μm-width microbeams were also confirmed using Gafchromic XR-RV3 radiochromic film (Ashland Inc., Covington, KY, USA). Scale bars, 1000, 500, and 200 μm.
