Table 1 Forward and reverse sequences for the primers used for RT-qPCR.
From: Transcriptomic and functional analyses of 3D placental extravillous trophoblast spheroids
Gene | Forward | Reverse | GenBank |
|---|---|---|---|
18S (RNA18S5) | CACGCCAGTACAAGATCCCA | AAGTGACGCAGCCCTCTATG | NR_003286.2 |
ACTB | TTACAGGAAGTCCCTTGCCATC | GCAATGCTATCACCTCCCCTG | NM_001101.5 |
GAPDH | TCACCATCTTCCAGGAGCGA | ATGACGAACATGGGGGCATC | NM_001357943.1 |
HIF1A | CAGCAACGACACAGAAACTGA | TTGGGTGAGGGGAGCATTAC | AF208487.1 |
VEGFA | GGCAGAATCATCACGAAGTGG | GGTCTCGATTGGATGGCAGT | NM_001171623.1 |
VEGFC | CCAATCACACTTCCTGCCGA | GCCTGACACTGTGGTAGTGTT | NM_005429.4 |
WNT5A | CTTGAGCACGACGAAGCAAC | GGAGGTTGGAGACAAAGGGG | NM_003392.4 |
TGFB1 | CTCCCGCAAAGACTTTTCCC | GAATAGGGGATCTGTGGCAGG | NM_000660.7 |
TGFB2 | TGCACCATGCTTTGGCTTTC | CTGGCTGGCTCAGCAACTAT | NM_001135599.3 |
CDH1 | ACACTGGTGCCATTTCCACT | TTAGGGCTGTGTACGTGCTG | AH006175.2 |
OCLN | TCGACCAATGCTCTCTCAGC | CTCCTGGAGGAGAGGTCCAT | U49184.1 |
TJP1 | TCTGAGCCTGTAAGAGAGGACT | GCTTGCTGCTTACCTGTTGAG | AF169196.1 |
ITGA2 | CGGTTATTCAGGCTCACCGA | ACCTACCAAGAGCACGTCTG | NM_002203.4 |
ITGA5 | CCAAAAGAAGCCCCCAGCTA | TCCTTGTGTGGCATCTGTCC | NM_002205.4 |
ITGB1 | AAGCGAAGGCATCCCTGAAA | GTCTACCAACACGCCCTTCA | NM_002211.4 |
VIM | GCAAAGACAGGCTTTAGCGAG | TTCAAGTCTCAGCGGGCTC | NM_003380.5 |
CLDN1 | CTGTCATTGGGGGTGCGATA | CTGGCATTGACTGGGGTCAT | NM_021101.5 |
CLDN4 | CCACTCGGACAACTTCCCAA | ACTTCCGTCCCTCCCCAATA | NM_001305.4 |
MMP9 | CCGGCATTCAGGGAGACGCC | TGGAACCACGACGCCCTTGC | NM_004994.2 |
TIMP2 | GAAGAGCCTGAACCACAGGT | GGGGGAGGAGATGTAGCAC | NM_003255.4 |
