Table 4 Results from hierarchical analyses of molecular variance (AMOVA) based on pairwise differences, on partial cytochrome-c-oxidase 1 (COI) sequences in Mediterranean Mytilicola intestinalis with mitochondrial Φ-statistic and conventional F-statistic.

	df	SS	VC	% var	p-value	Φ or F-statistic
*AMOVA 1: Gulf of Lion (Bouzigues, Istres, Rouet, Cassis) vs. Ligurian Sea (Livorno) vs. Adriatic Sea (Marjan, Malinska)*
among groups	2	0.476	−0.00303	−1.22	0.76653	Φ_CT = −0.01224
among populations within groups	4	1.671	0.00583	2.35	0.04089	Φ_SC = 0.02325
within populations	201	49.199	0.24477	98.87	0.04465	Φ_ST = 0.01130
total	207	51.346	0.24757
*AMOVA 1: Gulf of Lion (Bouzigues, Istres, Rouet, Cassis) vs. Ligurian Sea (Livorno) vs. Adriatic Sea (Marjan, Malinska)*
among groups	2	0.776	0.00261	1.54	0.20634	F_CT = 0.01543
among populations within groups	4	0.931	0.00230	1.36	0.13525	F_SC = 0.01378
within populations	201	33.062	0.16449	97.10	0.03040	F_ST = 0.02899
total	207	34.769	0.16940
*AMOVA 2: Gulf of Lion (Bouzigues, Istres, Rouet) vs. Ligurian Sea (Cassis, Livorno) vs. Adriatic Sea (Marjan, Malinska)*
among groups	2	0.801	0.00094	0.38	0.33257	Φ_CT = 0.00378
among populations within groups	4	1.346	0.00309	1.24	0.14168	Φ_SC = 0.01247
within populations	201	49.199	0.24477	98.38	0.04802	Φ_ST = 0.011620
total	207	51.346	0.24880
*AMOVA 2: Gulf of Lion (Bouzigues, Istres, Rouet) vs. Ligurian Sea (Cassis, Livorno) vs. Adriatic Sea (Marjan, Malinska)*
among groups	2	0.887	0.00350	2.07	0.16406	F_CT = 0.02069
among populations within groups	4	0.821	0.00137	0.84	0.23287	F_SC = 0.00825
within populations	201	33.062	0.16449	97.12	0.03168	F_ST = 0.02878
total	207	34.769	0.16936

Only samples with more than 15 individuals were included; see column N_parasites in Table 1. Abbreviations: df = degrees of freedom; SS = sum of squares; VC = variance component, % var = percentage of variation.

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