Figure 4
From: ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells
Expression of some ADAM15 isoforms leads to upregulation of claudin-1 in breast cancer cells. (a) Total protein extracts of MDA-MB-231 cells (n = 3) and (b) Total protein extracts of MCF-7 cells expressing ADAM15 isoforms grown to near-confluence were analysed for the expression of claudin-1. (c) Expression of the tight junction proteins ZO1 (n = 3), ZO2 (n = 1) and occludin (n = 5) in MDA/ADAM15 isoform expressing cells. (d) Stable downregulation of ADAM15 either by an shRNA to the coding region in MDA-MB-231 ADAM15A expressing cells or by an shRNA to the 3′UTR in T47D cells leads to a decrease in claudin1 expression. Dunnett’s multiple comparison test was carried out to assess significance. Confidence intervals: *p < 0.05; **p < 0.01; ***p < 0.001.
