Table 3 Comparison of pharmacology for test compounds in the GluA1o-γ4 intracellular calcium assays. Data are expressed as the mean ± standard deviation of n = 3 individual concentration-response experiments.
From: High-throughput-compatible assays using a genetically-encoded calcium indicator
Name | Type | GCaMP6s pEC50 | Fluo-4 pEC50 |
|---|---|---|---|
kainate | agonist | 5.84 ± 0.16 | 6.10 ± 0.09 |
glutamate | agonist | 5.41 ± 0.03 | 6.27 ± 0.03 |
domoate | agonist | 7.00 ± 0.12 | 7.34 ± 0.11 |
(S)-AMPA | agonist | 6.26 ± 0.03 | 6.99 ± 0.14 |
(R)-AMPA | agonist | 5.11 ± 0.13 | 5.81 ± 0.12 |
cyclothiazide | PAM | 5.31 ± 0.05 | 5.02 ± 0.25 |
LY395153 | PAM | 7.08 ± 0.04 | 6.99 ± 0.26 |
GCaMP6s pIC 50 | Fluo-4 pIC 50 | ||
CP-465022 | antagonist | 6.73 ± 0.33 | 6.77 ± 0.15 |
perampanel | antagonist | 7.10 ± 0.14 | 7.04 ± 0.10 |
talampanel | antagonist | 6.02 ± 0.27 | 5.65 ± 0.08 |
NBQX | antagonist | 6.14 ± 0.25 | 5.18 ± 0.06 |
GYKI53655 | antagonist | 6.16 ± 0.10 | 6.00 ± 0.13 |
