Figure 4
Miltefosine interfered with in vitro and in vivo capsaicin-induced TRPV1 activation. (A) Intracellular free calcium levels in response to different dosages of capsaicin in wildtype- and TRPV-1 transfected SH-SY5Y human neuroblastoma cells. (B) Capsaicin-induced activation of SH-SY5YhTRPV1 cells in the presence of a specific TRPV1 antagonist (SB-705498). (C) Capsaicin-induced activation of SH-SY5YhTRPV1 cells, pre-incubated with different dosages of miltefosine (mean +/− SD, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA, Dunnett’s post hoc test). (D) Schematic representation of experiments performed in the intracolonic capsaicin model and results of colorectal distensions in this model. Results are given as area under the curve of the relative response to distension (mean +/− SD, n = 9–10, ***P < 0.001, Repeated Measures one-way ANOVA, Sidak’s post hoc test).
