Figure 3
From: Modular Protein Ligation: A New Paradigm as a Reagent Platform for Pre-Clinical Drug Discovery
EPL Enables Alternative strategy for C-term peptide labeling. (a) SDS-PAGE and LC/MS analysis of murine Keap1 (322–624)-lanthanide generated via classical construct design with lanthanide peptide engineered into expression construct. (b) SDS-PAGE and LC/MS analyses of murine Keap1(322–624) with C-term lanthanide peptide or 10His peptide added via EPL (c) Regeneration of SPR chip enabled with murine Keap1(322–624)-Cys10His (d) High throughput binding kinetics of small molecules enabled by SPR; each blue circle represents a distinct compound. Several compounds had koff rates that were measured to be less than 1e-4, hence they could not be reliably fit and appear “pegged” to the y-axis.
