Table 1 Summary of MPL generated protein reagents across various target classes.
From: Modular Protein Ligation: A New Paradigm as a Reagent Platform for Pre-Clinical Drug Discovery
E. coli Expression - Intracellular Proteins | Modification | Potential End Use | Yield Final Product |
|---|---|---|---|
IDO1 (Uniprot P14902) | Biotin | ELT/SPR | 0.24 mg/g |
Cy5 | MST/cmpd aggr. | ~0.2 mg/g | |
none | Activity | 0.6 mg/g | |
murine Keap1(Uniprot Q9Z2X8) | lanthanide | NMR |  > 5 mg/g |
Biotin | ELT/SPR | ~1 mg/g | |
10His | ELT/SPR | ~1 mg/g | |
Fluorescein | MST/cmpd aggr. | ~1.5 mg/g | |
E. coli LpxC (Uniprot P0A725) | Biotin | ELT/SPR |  > 0.4 mg/g |
none | Activity | ~0.4 mg/g | |
P. aeruginosa LpxC (Uniprot P47205) | dual Strep | SPR | ~0.5 mg/g |
Biotin | ELT/SPR | ~0.5 mg/g | |
Ogg1 expressed with GyrA (Uniprot O15527) | Fluorescein | MST/cmpd aggr. | 2.25 mg/g |
Biotin | ELT/SPR | 2.6 mg/g | |
None | unlabeled control | 2.3 mg/g | |
Sirt1(1–747) (Uniprot Q96EB6) | Biotin | ELT/SPR |  > 0.14 mg/g |
Fluorescein | MST/cmpd aggr. |  > 0.14 mg/g | |
STING (149–379) H232R (Uniprot Q86WV6) | Biotin | ELT/SPR |  > 0.2 mg/g |
Fluorescein | MST/cmpd aggr. |  > 0.1 mg/g | |
Baculoviral Expression - Intracellular Protein | |||
Sirt1(183–664) expressed with VMA | Biotin | ELT/SPR | 1.5 mg/g |
Fluorescein | MST/cmpd aggr. | 1.0 mg/g | |
Sirt1(183–664) expressed with GyrA | Biotin | ELT/SPR | 1.3 mg/g |
Fluorescein | MST/cmpd aggr. | 1.3 mg/g | |
RIPK1 (Uniprot Q13546) | Biotin | ELT/SPR | ~0.01 mg/g |
lanthanide | NMR | ~0.04 mg/g | |
none | control | ~0.01 mg/g | |
Baculoviral Expression – Secreted | |||
TFR1 (L122-F760) (Uniprot P02786) | Biotin | SPR | 0.05–0.16 mg/L |
Fluorescein | MST/cmpd aggr. | 0.14 mg/L | |
Mammalian Expression of Secreted Protein | |||
soluble CD73 (Uniprot P21589) | Fluorescein | MST/cmpd aggr. |  > 5.0 mg/L Medium |
Biotin | ELT/SPR |  > 5.0 mg/L | |
Dansyl | Trp Fl | Trp Fl | |
Cy5 | MST | MST | |
10His | SPR | SPR | |
None | Activity | ~1.0 mg/L | |
IGD domain of aggrecan (Uniprot P16112) | Biotin | SPR | ~1.0 mg/L |
