Figure 3

Metabolite profiles of 15N-labeled Asp, Gly, Leu and Ser added to LOUCY cells, as assayed by 15N-edited HSQC of the media at time = 0 (a), the media at time = 60 min (b) and the cell extract at time = 60 min (c). Amino acid labels correspond to the one letter code. In all experiments, the buffer conditions were 100 mM 2H-acetate/pH 4.0, 10% 2H2O, 20% 2H-acetone.