Figure 2 | Scientific Reports

Figure 2

From: Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery

Figure 2

Phage display versus TEM1 antigen format. Raw ELISA profiling data for 30 randomly selected scFv hit clones isolated from the dCI h/mTEM1-SpyC antigen panning experiments. Bar length reflects the relative ELISA signal strengths for human and murine TEM1-SpyC and recombinant, CHO-cell derived and purified TEM1 full-length ECD antigens (L). ELISAs were conducted separately and independently for the TEM1-SpyC and TEM1 (L) antigens. Identical clone sequences obtained from 21 randomly picked hTEM1-bio R2 ELISA hits are shaded. SpyC antigens were captured via dCI on prepared streptavidin-bSpyT plate well surfaces whereas purified recombinant TEM1 (L) was immobilized on plate wells by passive adsorption.

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