Figure 1 | Scientific Reports

Figure 1

From: Genomic and developmental characterisation of a novel bunyavirus infecting the crustacean Carcinus maenas

Figure 1

Electron micrographs of the developmental cycle for ‘Carcinus maenas Portunibunyavirus 1’. (A) Single membrane viruses exit/enter the cell via cytosis of the cell membrane (black arrows) and enter the cell with a double membrane (white arrow) vesicles (V) including developing viral particles are present in section. (B) A lower magnification image shows an infected cell, among adjacent cells, with multiple vesicles containing developing virions. The nucleus (N) of an adjacent cell can be identified. (C) An image of an early developing vacuole with electron dense material, predicted to be viral RNA undergoing translation/transcription. (D) Virions are assembled. Vacuoles with late stage virions (V1) and pre-full-assembly virions are present in section (V2). (E) In some infected cells a paracrystalline array was noted to form in the cytoplasm of the host. F) As virions leave the cell, they shed their second membrane (black arrow). (G) Finally, infective virions enter the interstitial space between cells and aggregate before moving on to adjacent cells.

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