Figure 2
A graphical representation of the putative viral development for ‘Carcinus maenas Portunibunyavirus 1’ based on transmission electron micrographs attained from infected gill tissue. Infective stages enter the cell through endocytosis (1/Fig. 1A), providing them with a second membrane. This membrane is then predicted to shed (2), allowing release of viral -ssRNA. The genetic material then interacts with the cell, possibly through multiple pathways yet to be defined (3) but result in a series of large vacuoles where viral assembly occurs (4/Fig. 1C). After assembly, viral particles move from the assembly vesicle (5/Fig. 1D,G) and exit the cell (6/Fig. 1A). In addition to the classical bunyaviral development cycle, multiple occurrences of viroplasm development occur, suggesting a build-up of virions in the cytoplasm of the host cell which may rupture to result in virion release (“VIROPLASM”) (Fig. 1E). The diagram is not to cellular scale.
