Figure 5
From: An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture
Assessment of cell viability in restricted nutrient conditions after >72 hours in culture. P0 rat cardiac fibroblasts were seeded onto substrata of various elastic moduli and cultured in F10 medium supplemented with 2% FBS for 4 days. Cell viability was assessed with Calcein-AM (green) staining, and cell death by ethidium homodimer (red). Each biological replicate was assessed by capturing 3 random fields at 10X magnification. Images are representative of n = 3 biological replicates, with 9 technical replicates each.
