Table 1 List of primer pairs used in quantitative PCR.
From: An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture
Gene | Accession | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|---|
Acta2 | NM_031004.2 | AGATCGTCCGTGACATCAAGG | TCATTCCCGATGGTGATCAC |
Col1a1 | NM_053304.1 | TGCTCCTCTTAGGGGCCA | CGTCTCACCATTAGGGACCCT |
Col1a2 | NM_053356.1 | TGACCAGCCTCGCTCACAG | CAATCCAGTAGTAATCGCTCTTCCA |
Fn1 | NM_019143.2 | ACTGCAGTGACCAACATTGACC | CACCCTGTACCTGGAAACTTGC |
Hprt1 | NM_012583.2 | CTCATGGACTGATTATGGACAGGAC | GCAGGTCAGCAAAGAACTTATAGCC |
Postn | NM_001108550.1 | GCTTCAGAAGCCACTTTGTC | CGCCAACTACATCGACAAGG |
Tcf21 | NM_001032397.1 | CATTCACCCAGTCAACCTGA | CCACTTCCTTTAGGTCACTCTC |
