Table 1 Oligonucleotides used to determine relative TLR7 and TLR8 copy numbers by real-time quantitative PCR assays.
Oligonucleotide | Sequence |
|---|---|
TLR7 Forward | 5′-CAGTATTGTGCTGTCTTTGAAATGTA-3′ |
TLR7 Reverse | 5′-TGGTTGAAGAGAGCAGAGCA-3′ |
TLR7 Probe | 5′-(FAM)TTGGGCCCATCTCAAGCTGATCTTG(TAMRA)-3′ |
TLR8 Forward | 5′-GTGAGGCCACACAAGATGGA-3′ |
TLR8 Reverse | 5′-TTCCAGACCACTCCCTTTGC-3′ |
TLR8 Probe | 5′-(FAM)CGCCCAAGTGTCCACCTAAACATGAGT(TAMRA)-3′ |
HBD Forward | 5′-AGATTCCTACTTTCAGCGTTGG-3′ |
HBD Reverse | 5′-CAGCAGGGTTCAGGAAGATAAA-3′ |
HBD Probe | 5′-(FAM)CAACCTGGATCCACTTGCCCAGTG(TAMRA)-3′ |
RPP30 Forward | 5′-TTGTCGTTCAGAAGAAGACAAAGA-3’ |
RPP30 Reverse | 5′-AGTTGACTAGGGATTCGGAGAAA-3′ |
RPP30 Probe | 5′-(FAM)TGTTGATTTCAACACACAAATTCTGGTGG(TAMRA)-3′ |
