Figure 2 | Scientific Reports

Figure 2

From: Generation of Transmission-Competent Human Malaria Parasites with Chromosomally-Integrated Fluorescent Reporters

Figure 2

Passage through the mosquito of P. falciparum-tdTomato lines is normal. (A) Mosquito midguts (whitelight) were dissected 10 days after the infectious blood meal and imaged using fluorescence microscopy to visualize associated Pf-peg4-tdTomato and Pf-ef1α-tdTomato oocysts (red). (B) Oocysts were counted based on their tdTomato fluorescence and data from two independent feeding experiments each are shown. Horizontal line shows median oocyst number. Groups were compared using an unpaired t-test with equal standard deviations. (C) Pf-peg4-tdTomato and Pf-ef1α-tdTomato lines produce normal number of salivary gland sporozoites. Shown is the mean +/− standard deviation of salivary gland loads from 20 mosquitoes.

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