Figure 3 | Scientific Reports

Figure 3

From: Generation of Transmission-Competent Human Malaria Parasites with Chromosomally-Integrated Fluorescent Reporters

Figure 3

Immunofluorescence of sporozoites of P. falciparum-tdTomato lines. (A) The thorax of mosquitoes infected with the Pf-ef1α-tdTomato or Pf-peg4-tdTomato lines was visualized under a fluorescence stereoscope to detect the presence of the tdTomato sporozoites in the salivary glands. Note the red fluorescence observed in the thorax of mosquitoes infected with the P. falciparum-tdTomato lines compared to the absence of fluoresce in the uninfected mosquito. (B) Salivary gland sporozoites from mosquitoes infected with the Pf-ef1α-tdTomato or Pf-peg4-tdTomato were fixed and stained with an anti-PfCSP antibody (green) and analyzed by fluorescence microscopy to detect the cytoplasmic tdTomato (red) and the surface CSP (green). DNA was stained with DAPI (blue). Note the more intense signal detected from Pf-peg4-tdTomato sporozoites as compared to Pf-ef1α-tdTomato sporozoites, which match the fluorescence intensities from panel A.

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