Figure 1
Multiscreen evolution model set-up and experiment overview. (A) The bottom transport receiver plate was sterilized by exposure to UV-light and contained either medium (control condition) or a mixed bacterial community of S. aureus, S. anginosus, A. xylosoxidans, R. mucilaginosa, and G. haemolysans (microbiome condition). The sterile (0.22 µm PDFV) filter plate contained P. aeruginosa AA2 and was placed in the receiver plate, with the filters being emerged in the medium. A sterile lid secluded the set-up. (B) Pa AA2: P. aeruginosa AA2. Three independent overnight cultures were used to start three independent evolution samples = lineages (L1, L2, L3). For each lineage, P. aeruginosa biofilms were evolved in the absence (control) or presence of the CF microbial community (microbiome). Sa: S. aureus, Sag: S. anginosus, Ax: A. xylosoxidans, Rm: R. mucilaginosa, and Gh: G. haemolysans. T0 is the planktonic overnight culture at the start of the experiment, T1 (timepoint 1) is the P. aeruginosa biofilm (BF) obtained after 72 hours of microaerobic growth. 18 cycles of 72 hours ended in T18 (timepoint 18) after 54 days.
