Figure 2
Quantification of 3-O-C12-HSL production using an E. coli biosensor strain (expressing GFP in response to this molecule). The data is presented as delta fluorescence, by measuring the difference in fluorescence at 24 h compared to the start of the experiment, to normalize for autofluorescence. Graphs show means, error bars indicate standard deviations. n = 3, *p ≤ 0.05. T0: timepoint 0, planktonic culture. T1: timepoint 1, 72 h biofilm cells. T18: timepoint 18, biofilm cells after 18 cycles (54 days). L1: lineage 1, L2: lineage 2, L3: lineage 3. C: control P. aeruginosa evolved without microbiome, M: P. aeruginosa evolved with microbiome. Neg. ctrl: negative control, pure E. coli culture.
