Figure 4 | Scientific Reports

Figure 4

From: In vitro evolution of Pseudomonas aeruginosa AA2 biofilms in the presence of cystic fibrosis lung microbiome members

Figure 4

Pyocyanin production (A) and protease activity (B) of 2 sets of single isolates (C1 and C2: ΔlasRΔpqsR. C3 and C9: ΔlasR) from an evolved strain without microbiome presence (T18 L2 ctrl), selected depending on the presence and absence, respectively, of a mutation in pqsR (determined via Sanger sequencing). All isolates contained a mutation in lasR. Graphs show means, error bars indicate standard deviations. n ≥ 3, *p ≤ 0.05. (C) Photos of isolates C1, C2, C3, and C9 on LB agar showing the visible colour difference in the single isolates containing the different mutations.

Back to article page