Figure 1

Comparison of DNA extraction kits for preparing DNA libraries to detect pathogen-derived reads. DNA libraries were prepared using DNA extracted with the QIAamp DNA Microbiome kit (MB kit) or the QIAamp UCP Pathogen Mini kit (Path kit) from bronchoalveolar lavage fluid (BALF) samples. The composition of sequence data in each DNA library was compared. Each bar represents the composition of sequence reads including adapter, trimmed reads, human genome, bacteria, virus, eukaryotes, and others (A). Numbers of sequencing reads assigned to each bacterial species or cytomegalovirus (CMV) are shown (B). RPM: reads per million.