Figure 5
From: G-CSF and GM-CSF Modify Neutrophil Functions at Concentrations found in Cystic Fibrosis
Effect of GM-CSF and G-CSF on neutrophil chemotaxis. (A) Neutrophils were allowed to migrate for 2.5 h in the absence or presence of either GM-CSF or G-CSF at the indicated concentrations. fMLP (1 μM) was used a positive control. (B) Neutrophils were pre-treated with either CSF for 50 min and allowed to migrate along a fMLP gradient for 2.5 h. (C) Neutrophils were either allowed to migrate for 2.5 h in the absence or presence of both CSFs contemporarily presented in the lower chamber at low concentrations (0.005 or 0.1 ng/ml) and with fMLP as positive control, or first pre-treated with both CSFs contemporarily for 50 min at the same concentrations and then allowed to migrate along a fMLP gradient for 2.5 h. Data are shown as percentage of migrated neutrophils as compared with total neutrophils added in the apical compartment. Results are shown as mean ± SEM of six experiments. ANOVA (with Tukey’s post hoc test) and Student t-test. In all panels, ***P < 0.0001: +fMLP controls vs. -fMLP controls; *P < 0.05, **P < 0.01, and ***P < 0.0001 as compared with untreated and –fMLP controls or untreated and +fMLP controls.
