Figure 4 | Scientific Reports

Figure 4

From: High Impact Exercise Improves Bone Microstructure and Strength in Growing Rats

Figure 4

Impact loading setup and strain gauge calibration. (A) With the rats under anesthesia, the right tibiae from LI, MI and HI groups were loaded using a waveform generating 450, 850, and 1250 µε at the medio-proximal tibial surface from the 4 to 11 week period. (B) The loading profile consisted of haversine waveform displacements at 2 Hz and characterized by symmetric loading/unloading with a 0.10 sec of rest insertion between loading cycles. Loadings were repeated for 1200 cycles, yielding a daily (5 days/week) loading period of 10 minutes. (C) Strain gauge positioned at the medio-proximal surface of the tibia for allowing strain assessment for 0.5 mm to 3.5 mm of displacement. (D) Linear relationship between applied displacement and resulting strain at the medio-proximal surface of 4, 8 and 12 week old rat tibiae (mean value ± SD) (N = 6 rats/group).

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