Figure 4
From: High Impact Exercise Improves Bone Microstructure and Strength in Growing Rats

Impact loading setup and strain gauge calibration. (A) With the rats under anesthesia, the right tibiae from LI, MI and HI groups were loaded using a waveform generating 450, 850, and 1250 µε at the medio-proximal tibial surface from the 4 to 11 week period. (B) The loading profile consisted of haversine waveform displacements at 2 Hz and characterized by symmetric loading/unloading with a 0.10 sec of rest insertion between loading cycles. Loadings were repeated for 1200 cycles, yielding a daily (5 days/week) loading period of 10 minutes. (C) Strain gauge positioned at the medio-proximal surface of the tibia for allowing strain assessment for 0.5 mm to 3.5 mm of displacement. (D) Linear relationship between applied displacement and resulting strain at the medio-proximal surface of 4, 8 and 12 week old rat tibiae (mean value ± SD) (N = 6 rats/group).