Figure 4

FAM83A loss induces apoptotic cell death. (a–c) HPAC-shGFP, HPAC-shA2 and HPAC-shA6 cells were assessed for proliferation and apoptosis over four days. Cells were labelled with CFSE and cellular proliferation was assessed by the dilution of CFSE at 0, 24, 48 and 72 h shown as overlaid histogram plots (a). Cells were stained with DNA-binding dye propidium iodide for cell cycle analysis by flow cytometry. The histogram plots in b show the sub-G1, G0-G1 and G2-M population at 96 h. (c) Cellular apoptosis was assessed by Annexin V and PI staining. The plots show increased numbers of apoptotic (Annexin V⁺) and dead (Annexin V⁺PI⁺) cells in the shA groups at 48 h. All data are representative of three independent experiments.