Figure 4

Representative immunoblots from ZIKV neutralizing antibody negative (ZIKV PRNT < 20) serum. Supernatant was collected from mock (M) and flavivirus (ZIKV (Z), JEV (J) and DENV 1-4 (D1-D4)) infected BHK-21 cells. Proteins were separated by SDS-PAGE under native conditions and transferred onto nitrocellulose membranes. Membranes were probed with ZIKV neutralizing antibody negative (ZIKV PRNT < 20) serum. Panel A shows the immunoblot of serum sample S6, while panel B shown the immunoblot for serum sample S60. The asterisk (*) marks the position of the NS1 dimer. PRNT₉₀ values are as reported previously⁸. An additional 28 immunoblots can be found in the Supplemental materials file.