Figure 4

Effects of roscovitine and IPC on Rb expression after TCI. (A) Western blots of total Rb and p-Rb in the CA1 area of the TCI, roscovitine + TCI and IPC + TCI groups at sham, 1, 2 and 5 days after TCI. β-actin is used as a protein loading control. Relative band intensity of Rb and p-Rb level is measured by densitometer. p-Rb level is significantly low in the roscovitine + TCI and IPC + TCI groups compared with the TCI group. Molecular weight is indicated as kDa on the right side of the immunoblots. The bars are reported as means ± SEM from three independent experiments (n = 7, ^*P < 0.05 vs. sham group; ^#P < 0.05 vs TCI group; ^†P < 0.05 vs roscovitine + TCI group). (B) p-Rb immunoreactivity in the CA1 area of the TCI (left column), roscovitine + TCI (middle column) and IPC + TCI (right column) groups at sham, 1, 2 and 5 days after TCI. p-Rb immunoreactivity in the TCI group is very strong in nuclei (arrows) of CA1 pyramidal neurons 1 and 2 days after TCI. In the roscovitine + TCI and IPC + TCI groups, the pattern of p-Rb immunoreactivity in CA1 pyramidal neurons was similar to that in the sham group. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bar = 50 µm. Quantitative graph of p-Rb immunoreactivity in CA1 pyramidal neurons. A ratio of the ROD was calibrated as %, with the sham group designated as 100%. The bars are reported as means ± SEM from three independent experiments (n = 7, ^*P < 0.05 vs. sham group; ^#P < 0.05 vs TCI group; ^†P < 0.05 vs roscovitine + TCI group).