Figure 5
Effects of roscovitine and IPC on p53 phosphorylation and its translocation after TCI. (A) Western blots of p53 and p-p53 in the CA1 area of the TCI, roscovitine + TCI and IPC + TCI groups at sham, 1, 2 and 5 days after TCI. β-actin is used as a protein loading control. Relative band intensity of total p53 and p-p53 level is measured by densitometer. p-p53 levels are significantly low in the roscovitine + TCI and IPC + TCI groups compared with the TCI group. Molecular weight is indicated as kDa on the right side of the immunoblots. The bars are reported as means ± SEM from three independent experiments (n = 7, *P < 0.05 vs. sham group; #P < 0.05 vs TCI group; †P < 0.05 vs roscovitine + TCI group). (B) p-p53 immunoreactivity in the CA1 area of the TCI (left column), roscovitine + TCI (middle column) and IPC + TCI (right column) groups at sham, 1, 2 and 5 days after TCI. p-p53 immunoreactivity in the TCI group is very strong in nuclei (arrows) of CA1 pyramidal neurons 1 and 2 days after TCI. In the roscovitine + TCI and IPC + TCI groups, p-p53 immunoreactivity in CA1 pyramidal neurons is moderated 1 and 2 days after TCI, and the immunoreactivity is shown in both nuclei and cytoplasm. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bar = 50 µm. Quantitative graph of p-p53 immunoreactivity in CA1 pyramidal neurons. A ratio of the ROD was calibrated as %, with the sham group designated as 100%. The bars are reported as means ± SEM from three independent experiments (n = 7, *P < 0.05 vs. sham group; #P < 0.05 vs TCI group; †P < 0.05 vs roscovitine + TCI group).
