Figure 3

Time-dependent changes in Ca²⁺ transient (single-cell analysis) during maturation. (A) Representative line scan images with corresponding spatially averaged profiles of cytosolic Ca²⁺ transients evoked by electrical field stimulation at 0.5 Hz in cells cultured for Wk-1, Wk-2, and Wk-4, respectively. (B) Average values for systolic and end decay of Ca²⁺ cyt. (C) Time to Ca²⁺ transient peaks. (D) Rate of decay of Ca²⁺ transients recorded in Wk-1 (n = 51–59), Wk-2 (n = 41–46), and Wk-4 (n = 71–76), where ‘n’ indicates number of cells. *p < 0.05 vs Wk-1, **p < 0.01 vs Wk-1, ^§p < 0.05 vs Wk-2, ^§§p < 0.001 vs Wk-2 (t-tests with Bonferroni correction).