Figure 2
Sensorgram for optimization of antibody immobilization using different concentrations of primary capture antibody (goat F(ab) anti-rabbit IgG H&L antibody). (a) Sensor responses obtained due to the binding of 100 µg mL−1 of the secondary a rabbit polyclonal antibody with specificity to C. jejuni. (b) Comparison of the binding response obtained from 3 and 4 minutes of injection period of the primary capture antibody during the immobilization process. (c) Error bars represent the mean ± standard deviation of triplicates.
