Figure 3
Extracellular DNA quantification from ZIKV-infected human neutrophils. (A–C) Human neutrophils (1 × 105/300 μL) were stimulated with PMA (100 nM) or infected with ZIKV (MOI 2) for 6 hours in 8-chamber culture. Cells were stained for DNA with Hoechst 33342. Scale bars = 100 μm, Magnification = 20×. (D,E) Neutrophils (2 × 106/mL) were stimulated with ZIKV, UV-ZIKV or PMA or left unstimulated for 6 hours. Extracellular DNA (μg/mL) was quantified in culture supernatants and fold number of concentrations was evaluated using the negative control (NCrl) as reference. Data are representative of 2 independent experiments performed in triplicates and represent mean ± SD. Data were analyzed with Kruskal-Wallis test. *p < 0.05, **p < 0.01.
