Figure 5
From: All-optical Reflection-mode Microscopic Histology of Unstained Human Tissues
A comparison between a PARS images and (a) a bright field image at the of the same breast tissue sample with invasive ductal carcinoma. (b) an unstained slide imaged with a conventional bright field microscope. A PARS acquisition of the regions is shown for the unstained tissue slide with (c) a false H&E colour map applied and a (d) logarithmic greyscale colour map applied. A PARS acquisition of a FFPE block with (e) a false H&E colour map applied and (f) a logarithmic greyscale colour map applied. Green outline highlights a sharp delineation between a hypercellular region of tissue and healthy tissue, blue outline represents a high-grade malignancy with serosity and necrotic cells, the red outline represents an artifact present in the slides. Scale bar 100 µm.
