Figure 2
Fluorescent immunohistochemical staining of MBP with signal intensity assessment. In the brainstem, very pronounce differences in MBP labeling intensity were observed between WT EAE (n = 3) (a) and Cpt1a P479L EAE mice (n = 3). Particularly, intense signal was noted the ventral spinocerebellar tract, the pyramid and inferior olivary complexes. Labeling of these structures in WT EAE mice shown markedly weaker intensity. Similarly, in the cerebellum (illustrated on cerebral cortex, simple lobule), more intense MBP labeling was noted in the region of arbor vitae in the form of fibrous structures protruding into the granular cerebellar layer in Cpt1a P479L EAE mice. Whereas in the cerebellum of WT EAE mice, MBP labeling was noticeably less intense. Labeling intensity quantification further confirmed the observed differences in MBP expression in the brainstem and in the cerebellum between WT EAE and Cpt1a P479L EAE (unpaired t test) (e). Scale bar: 100 µm. All images acquired with identical settings at approximately −6.5 mm ant. bergma. Data are presented as the mean ± SEM. Asterisks indicate the level of significance (*p < 0.05, **p < 0.01).
