Figure 2
From: Dopamine neuron induction and the neuroprotective effects of thyroid hormone derivatives
The neurogenic effect of FBS is NURR1-dependent. (A) To test whether FBS induces general neurogenesis, the expression of the neuronal-specific marker MAP2 in Nurr1-overexpressing rat NPCs on differentiation day 7 was determined. More TH+ cells were found in FBS-treated cultures, although there was also a significant increase in the number of MAP2+ cells. (B) FBS has a neurogenic effect only in the presence of NURR1. The number of MAP2+ cells did not increase in the absence of Nurr1 overexpression. FBS induced an increase in the number of GFAP+ cells in both Nurr1 retrovirus-transduced and nontransduced cultures. An anti-GFAP antibody was used as an astrocyte-specific marker. **p < 0.01. Scale bar, 20 μm. The error bars are the S.E. from two independent experiments.
