Figure 6

Thyroid hormones and their derivatives can protect DA neurons from neurotoxic damage. (A) Timeline of the experimental protocol for the dopaminergic neuron protection test. (B) Nurr1-Mash1-overexpressing rat NPCs were treated with thyroid hormones or their derivatives for 24 h on differentiation day 5. The following day, a neurotoxic insult (6-OHDA for 18 h or H₂O₂ for 3 h) was delivered to the DA neurons. On differentiation day 7, DA neurons were immunostained with an anti-TH antibody. TH+ neurons were detected following treatment with both the thyroid hormones and their derivatives. **p < 0.01. Scale bar, 20 μm. The error bars are the S.E. from two independent experiments.