Figure 2
From: Random transposon insertion in the Mycoplasma hominis minimal genome
Insertion site of the tet(M) gene in the genome of the M. hominis M132 transformant 28-2. (A) Scheme of the transposon inserted into the M. hominis M132 genome. The region contains inverted repeats (point rectangles), the sequence of the pMT85 plasmid (white rectangles) and the tet(M) gene (dark gray rectangle). (B) Scheme of insertion site for transformant 28-2. Hatched rectangles represent the M. hominis M132 gene where the tet(M) gene inserted. Double thin lines represent the position of the insertion inside the M. hominis M132 gene. Clear gray rectangles correspond to sequence of the bacterial genome. Numbers above single thin lines indicate the position around the genome. Black arrows indicate the position of the PCR primers P75-F1 and P75-R1, and gray arrows indicate the position of the PCR primers P75-F2 and P75-R2.
