Figure 2
Bone marrow-derived murine macrophages differentiated with GM-CSF show a trend towards a reduced expression of pattern recognition receptors upon RV infection while exhibiting increased expression of IRF4. Bone marrow derived monocytes were differentiated to macrophages by stimulation with either GM-CSF or M-CSF followed by infection with RV1B for 24 h. Gene expression of RIG-I (A), MDA5 (B), TLR3 (C) and IRF4 (E) was measured by real-time PCR and data is presented as mean ± standard deviation fold change of untreated M-CSF stimulated macrophages relative to HPRT expression. Comparison of different groups was performed by Kruskal-Wallis with Wilcoxon post testing. *p < 0.05, **p < 0.01, ***p < 0.001 vs. own CTRL if not stated otherwise. Data from 6 independent experiments. A representative Western Blot image of TLR3, RIG-I and MDA5 protein is shown (D). Blots were stripped after incubation with anti-TLR3 mAb and successively reprobed with anti-RIG-I mAb and anti-MDA5 mAb. Blots were run on the same gel and were not cropped.
