Table 1 Purification of OSM from MBP-OSM expressed in BL21(DE3) cells at 18 °C.

From: Prokaryotic soluble overexpression and purification of oncostatin M using a fusion approach and genetically engineered E. coli strains

Purification step

Total protein (mg)

Purity (%)a

OSM (mg)b

Yield (%)

Bacterial culture (500 mL)

2,500 (pellet)

—

—

—

Supernatant

155.7

29.5

15.4

100

1st IMAC eluate

51.2

89

15.2

98.7

2nd IMAC eluate

11.6

95

11.02

71.6

  1. aPurity of the protein of interest was determined by densitometry using ImageJ.
  2. bThe amount of OSM was calculated based on the purity of the protein of interest and the size correlation between OSM and the corresponding fusion protein.
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