Figure 1 | Scientific Reports

Figure 1

From: PCR-based Sepsis@Quick test is superior in comparison with blood culture for identification of sepsis-causative pathogens

Figure 1

Scheme of study design and workflow. (A) Former MCLB-1 based stepwise realtime PCR protocol including Group-specific screening by PCR reactions targeting bacterial 16SrRNA gene to differentiate Gram-positive, Gram-negative and Enterobacteriaceae groups. Samples positive in the screening assay were subjected to genus-specific real-time PCR reactions to detect 13 most common sepsis causative pathogens. (B) the current protocol of Sepsis@quick diagnostics kit was subjected to individual to genus-specific real-time PCR of E. coli, K. pneumoniae, P. auriginosa, A. baumannii, N. meningitidis, Staphylococus sp, S aureus, Streptococus sp. S. suis, S. pneumonia, Enterococcocus sp, Fusobacterium sp and Bacteriodes sp.

Back to article page