Figure 2
From: Targeted genomic CRISPR-Cas9 screen identifies MAP4K4 as essential for glioblastoma invasion
Validation of genes inhibiting migration using a wound healing assay. Eleven genes significantly enriched in the noninvasive population from the screen were validated in a wound healing assay. (A) Scratch area (red) is identified at 0, 12 and 24 hours in a representative monolayer of the specified cell line. (B) Quantification of normalized migration rate of each cell line calculated from 6–12 hours, n ≥ 7 for each condition, *p < 0.05, **p < 0.01, ***p < 0.001. (C) Average scratch area over time for the control U138-Cas9 cell line (solid black line +/− SD) and MAP4K4 knockout lines using two separate sgRNA sequences (red and blue lines +/− SD).
