Figure 6
From: Targeted genomic CRISPR-Cas9 screen identifies MAP4K4 as essential for glioblastoma invasion
MAP4K4 regulates EMT in U138 cells. Immunoblots showing that either (A) knocking-out MAP4K4 or (B) inhibiting MAP4K4 leads to a protein expression profile more consistent with a non-invasive state. E-cadherin expression increases while β-catenin, Slug, and Vimentin decrease. GAPDH serves as a control. For MAP4K4 inhibitor (B) cells were cultured in 5 μM of PF06260933 dihydrochloride for either 3 or 7 days. Immunoblots were cropped. Full size immunoblots can be found in supplementary materials.
